gondiioocysts from contaminated ground, undercooked meat containing the parasite cysts, direct contact with cat feces or by vertical transmission from mother to fetus, via the placenta.[1,2,8] Although human being toxoplasmosis is generally asymptomatic in a wide range of instances, it is of clinical importance when early Nutlin carboxylic acid infection happens or when there is a reactivation of infection in immunosuppressed individuals.[9,10,11] Toxoplasmosis commonly causes slight symptoms in immunocompetent individuals; whereas, in immunocompromised individuals it is often fatal. risk factors (Direct cat exposure, Occupational exposure to raw meat, and Raw vegetable consumption) were recorded. The enzyme-linked immunosorbent assay (ELISA) packages (EUROIMMUN, United Kingdom) were utilized for the evaluation of anti-toxoplasma IgG and IgM antibodies according to the manufacturer’s instructions. All analyses were carried out using SPSS-20. == Results: == The rate of recurrence of toxoplasma-specific IgG and IgM antibodies like: Direct cat exposures, Occupational exposure to raw meat, and Raw vegetable consumption were not statistically significant between the two organizations (P> 0.05). The history of earlier abortions in women in the toxoplasmosis-suspected group was significantly higher than that in the settings (31.4% versus 6.7%;P= 0.009). == Summary: == The rate of recurrence of specific IgM and IgG antibodies in toxoplasmosis suspected in the toxoplasmosis and control organizations was not statistically significant. Keywords:IgG antibody, IgM antibody, Isfahan,Toxoplasma gondii, toxoplasmaspecific antibodies == Intro == Toxoplasma gondiiis probably one of the most common intracellular protozoan parasites, with up to 30% Nutlin carboxylic acid of the world’s human population affected by this parasite and the third main cause of food-related deaths in the United States of America[1,2,3]. In Iran, the pace of toxoplasmosis, in 2008, was 40.7% for Isfahan, 44.2% for Lorestan, and 34.2% for Bandar-e-Abbas.[4] Albeit pet cats are the certain hosts, the parasite can infect approximately all mammals and parrots.[5,6,7] Human beings are infected mainly by ingestion ofT. gondiioocysts from contaminated soil, undercooked meat comprising the parasite cysts, direct contact with cat feces or by vertical transmission from mother to fetus, via the placenta.[1,2,8] Although human being toxoplasmosis is generally asymptomatic in a wide range of instances, it is of clinical importance when early infection happens or when there is a reactivation of infection in immunosuppressed individuals.[9,10,11] Toxoplasmosis commonly causes slight symptoms in immunocompetent individuals; whereas, in immunocompromised individuals it is often fatal. Acute toxoplasmosis in the pregnancy period can lead to abortion, neonatal death, and poor growth or delivery before time.[12,13] Histopathological assessment and serological procedures, including the dye test (DT), indirect fluorescent antibodies (IFA), altered agglutination test (MAT), and enzyme-linked immunosorbent assay (ELISA) are generally utilized for the diagnosis ofT. gondiiinfection.[14,15,16,17] Nowadays, a number of fresh methods are widely used for the analysis ofT. gondiiinfection in Europe. These methods are: The serum IgG avidity test, polymerase chain reaction (PCR) with body cells, and Western blots of serum.[18] Also the Real-time PCR (RT-PCR) is another method that has newly been introduced Nutlin carboxylic acid for the analysis of toxoplasmosis infection.[19,20] The majority of serological assays rely on anti-T. gondiitotal immunoglobulin antibodies in the serum specimens. IgM antibodies are the first class of antibodies that appear during a main illness withT. gondii.[21,22] Also, the IgG-avidity test has been used to help the diagnosis of acute infection, which has produced at little amount after early infection and offers increased over time.[23] Probably the most generally applied methods for the measurement of IgM and IgG antibodies are enzyme-linked immunosorbent assay kits.[21,24] The ELISA test for diagnosis of antibodies toT. gondiihas the potential to generate stable results in different laboratories, while using different packages.[25] All in all the presence and titration of IgM and IgG antibodies in individuals suspected with toxoplasmosis are not clearly understood. The present investigation seeks to determine the presence and titration of IgM and IgG antibodies in individuals suspected with toxoplasmosis, using ELISA. Furthermore, it also tries Sdc2 to find an association between IgM and IgG antibodies with some risk factors, such as, direct exposure to cats, occupational exposure to raw meat, and consumption of raw fruits and vegetables. == MATERIALS AND METHODS == The present comparative cross-sectional study was conducted during 2012, in Isfahan, Iran. Before initiation, the study protocol was approved by the Ethics Committee of the Isfahan University of Medical Sciences. Written informed consent was obtained from all participants after full explanation of the nature of the study. They received a description of the study procedures and potential risks and benefits. Seventy patients with suspected toxoplasmosis and 30 healthy volunteers, who exceeded informed consent, entered the study. In this study, the sample size was decided using the Krejcie and Morgan table. The non-probability consecutive sampling method was used. In each group, patient age, sex, signs of appearance, education level, residency status (urban/rural), occupation, frequency of toxoplasma-specific IgG and IgM antibodies, abortion history, and some risk factors (direct cat exposure, occupational exposure to raw meat, and raw vegetable consumption) were recorded. The ELISA kits (EUROIMMUN, United Kingdom) were used for the evaluation of anti-toxoplasma IgG and IgM Nutlin carboxylic acid antibodies according to the manufacturer’s instructions. In this study, toxoplasma-specific IgG antibodies with results greater than 11 IU / ml were considered to be positive. Also sera with titers of IgM >1.1 IU / ml were regarded as positive. The data were presented as Mean SD for continuous variables and Number (Percent) for categorical ones. Statistical differences among the.